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gene amp 7000 sequence detection system  (Thermo Fisher)


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    Structured Review

    Thermo Fisher gene amp 7000 sequence detection system
    Gene Amp 7000 Sequence Detection System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gene+amp+7000+sequence+detection+system/Ampicillin+trihydrate/pm38567476-78-6-13
    Average 99 stars, based on 1 article reviews
    gene amp 7000 sequence detection system - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Human ribosomes from cells with reduced dyskerin levels are intrinsically altered in translation.
    Article Snippet: Dyskerin is a pseudouridine (c) synthase involved in fundamental cellular processes including uridine modification in rRNA and small nuclear RNA and telomere stabilization.. Dyskerin functions are altered in X-linked dyskeratosis congenita (X-DC) and cancer.. Dyskerin’s role in rRNA pseudouridylation has been suggested to underlie the alterations in mRNA translation described in cells lacking dyskerin function, although relevant direct evidences are currently lacking.

    Article Title: A selective inhibition of c-Fos/activator protein-1 as a potential therapeutic target for intervertebral disc degeneration and associated pain
    Article Snippet: .. Gene expression was quantified using qPCR with a Gene Amp 7000 Sequence Detection System (Applied Biosystems, Warrington, UK). ..

    Article Title: Inhibition of human dyskerin as a new approach to target ribosome biogenesis.
    Article Snippet: .. Real-time PCR analysis was performed in a Gene Amp 7000 Sequence Detection System (Applied Biosystems) using the Syber-green (Applied Biosystems) approach. .. The set of primers (IDT DNA) used were: primerF 59- GAACCTGAACCGGCCAAG-39 and primerR 59-CTATAGGGCGTCGCTATGA-39.

    Article Title: Dyskerin and TERC expression may condition survival in lung cancer patients
    Article Snippet: Briefly, semi-quantitative Taqman approach (TaqMan Universal PCR master mix, Applied Biosystems) was employed to evaluate the expression of TERC, DKC1 and beta-glucoronidase as endogenous control. .. All real-time PCR reactions were carried out in triplicate in a Gene Amp 7000 Sequence Detection System (Applied Biosystems). ..

    Article Title: In vitro alteration of physiological parameters do not hamper the growth of human multipotent vascular wall-mesenchymal stem cells
    Article Snippet: .. Real Time PCR analysis was carried out in a Gene Amp 7000 Sequence Detection System (Applied Biosystems) using the TaqMan approach for the β-glucuronidase (GUS), B-cell lymphoma-2 (BCL-2), BCL-2 associated protein (BAX) genes (Applied Biosystems) and SYBR green approach for all other genes, using specific couples of primers, purchased from SIGMA-Aldrich: MMP-2 (FWD 5′-CCCCAAAACGGACAAAGAG-3′, REV 5′-CTTCAGCACAAACAGGTTGC-3, MMP-9 (FWD 5′-GAACCAATCTCACCGACCAG-3′, REV 5′-GCCACCCGAGTGTAACCAT-3′). ..

    Article Title: Acute telomerase components depletion triggers oxidative stress as an early event previous to telomeric shortening
    Article Snippet: .. The RT mixture was used as a template for the subsequent Real-time PCR analysis, which was performed in a Gene Amp 7000 Sequence Detection System (Applied Biosystems) by the Syber-green (Applied Biosystems) approach. ..

    Article Title: The crosstalk between vascular MSCs and inflammatory mediators determines the pro-calcific remodelling of human atherosclerotic aneurysm
    Article Snippet: One microgram of total RNA was reverse transcribed in a 20-μl reaction volume using a High Capacity Reverse Transcription Kit (Life Technologies). .. Real-time PCR was carried out in a Gene Amp 7000 Sequence Detection System (Applied Biosystems) using the SYBR green mix (Life Technologies) and specific couples of primers were designed using the NCBI BLAST tool (purchased from Sigma Aldrich; Table ). ..

    Sequencing:

    Article Title: Human ribosomes from cells with reduced dyskerin levels are intrinsically altered in translation.
    Article Snippet: Dyskerin is a pseudouridine (c) synthase involved in fundamental cellular processes including uridine modification in rRNA and small nuclear RNA and telomere stabilization.. Dyskerin functions are altered in X-linked dyskeratosis congenita (X-DC) and cancer.. Dyskerin’s role in rRNA pseudouridylation has been suggested to underlie the alterations in mRNA translation described in cells lacking dyskerin function, although relevant direct evidences are currently lacking.

    Article Title: A selective inhibition of c-Fos/activator protein-1 as a potential therapeutic target for intervertebral disc degeneration and associated pain
    Article Snippet: .. Gene expression was quantified using qPCR with a Gene Amp 7000 Sequence Detection System (Applied Biosystems, Warrington, UK). ..

    Article Title: Inhibition of human dyskerin as a new approach to target ribosome biogenesis.
    Article Snippet: .. Real-time PCR analysis was performed in a Gene Amp 7000 Sequence Detection System (Applied Biosystems) using the Syber-green (Applied Biosystems) approach. .. The set of primers (IDT DNA) used were: primerF 59- GAACCTGAACCGGCCAAG-39 and primerR 59-CTATAGGGCGTCGCTATGA-39.

    Article Title: Dyskerin and TERC expression may condition survival in lung cancer patients
    Article Snippet: Briefly, semi-quantitative Taqman approach (TaqMan Universal PCR master mix, Applied Biosystems) was employed to evaluate the expression of TERC, DKC1 and beta-glucoronidase as endogenous control. .. All real-time PCR reactions were carried out in triplicate in a Gene Amp 7000 Sequence Detection System (Applied Biosystems). ..

    Article Title: Impact of toll-like receptor 4 variations on nasopharyngeal carcinoma risk and survival in tunisian population.
    Article Snippet: This reaction was comprised of 20 μM of each primer and probe with 10 μl de Master Mix (Universal Master Mix, Applied Biosystems, (Foster City, CA, USA) and 50ng of DNA. .. PCR reactions were performed on a Gene Amp 7000 sequence detection System (AB Applied Biosystems, (Foster City, CA, USA). ..

    Article Title: In vitro alteration of physiological parameters do not hamper the growth of human multipotent vascular wall-mesenchymal stem cells
    Article Snippet: .. Real Time PCR analysis was carried out in a Gene Amp 7000 Sequence Detection System (Applied Biosystems) using the TaqMan approach for the β-glucuronidase (GUS), B-cell lymphoma-2 (BCL-2), BCL-2 associated protein (BAX) genes (Applied Biosystems) and SYBR green approach for all other genes, using specific couples of primers, purchased from SIGMA-Aldrich: MMP-2 (FWD 5′-CCCCAAAACGGACAAAGAG-3′, REV 5′-CTTCAGCACAAACAGGTTGC-3, MMP-9 (FWD 5′-GAACCAATCTCACCGACCAG-3′, REV 5′-GCCACCCGAGTGTAACCAT-3′). ..

    Article Title: Acute telomerase components depletion triggers oxidative stress as an early event previous to telomeric shortening
    Article Snippet: .. The RT mixture was used as a template for the subsequent Real-time PCR analysis, which was performed in a Gene Amp 7000 Sequence Detection System (Applied Biosystems) by the Syber-green (Applied Biosystems) approach. ..

    Article Title: The crosstalk between vascular MSCs and inflammatory mediators determines the pro-calcific remodelling of human atherosclerotic aneurysm
    Article Snippet: One microgram of total RNA was reverse transcribed in a 20-μl reaction volume using a High Capacity Reverse Transcription Kit (Life Technologies). .. Real-time PCR was carried out in a Gene Amp 7000 Sequence Detection System (Applied Biosystems) using the SYBR green mix (Life Technologies) and specific couples of primers were designed using the NCBI BLAST tool (purchased from Sigma Aldrich; Table ). ..

    Gene Expression:

    Article Title: A selective inhibition of c-Fos/activator protein-1 as a potential therapeutic target for intervertebral disc degeneration and associated pain
    Article Snippet: .. Gene expression was quantified using qPCR with a Gene Amp 7000 Sequence Detection System (Applied Biosystems, Warrington, UK). ..

    Polymerase Chain Reaction:

    Article Title: Impact of toll-like receptor 4 variations on nasopharyngeal carcinoma risk and survival in tunisian population.
    Article Snippet: This reaction was comprised of 20 μM of each primer and probe with 10 μl de Master Mix (Universal Master Mix, Applied Biosystems, (Foster City, CA, USA) and 50ng of DNA. .. PCR reactions were performed on a Gene Amp 7000 sequence detection System (AB Applied Biosystems, (Foster City, CA, USA). ..

    SYBR Green Assay:

    Article Title: In vitro alteration of physiological parameters do not hamper the growth of human multipotent vascular wall-mesenchymal stem cells
    Article Snippet: .. Real Time PCR analysis was carried out in a Gene Amp 7000 Sequence Detection System (Applied Biosystems) using the TaqMan approach for the β-glucuronidase (GUS), B-cell lymphoma-2 (BCL-2), BCL-2 associated protein (BAX) genes (Applied Biosystems) and SYBR green approach for all other genes, using specific couples of primers, purchased from SIGMA-Aldrich: MMP-2 (FWD 5′-CCCCAAAACGGACAAAGAG-3′, REV 5′-CTTCAGCACAAACAGGTTGC-3, MMP-9 (FWD 5′-GAACCAATCTCACCGACCAG-3′, REV 5′-GCCACCCGAGTGTAACCAT-3′). ..

    Article Title: The crosstalk between vascular MSCs and inflammatory mediators determines the pro-calcific remodelling of human atherosclerotic aneurysm
    Article Snippet: One microgram of total RNA was reverse transcribed in a 20-μl reaction volume using a High Capacity Reverse Transcription Kit (Life Technologies). .. Real-time PCR was carried out in a Gene Amp 7000 Sequence Detection System (Applied Biosystems) using the SYBR green mix (Life Technologies) and specific couples of primers were designed using the NCBI BLAST tool (purchased from Sigma Aldrich; Table ). ..



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    Thermo Fisher gene amp 7000 sequence detection system
    Gene Amp 7000 Sequence Detection System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gene+amp+7000+sequence+detection+system/Ampicillin+trihydrate/pm38567476-78-6-13
    Average 99 stars, based on 1 article reviews
    gene amp 7000 sequence detection system - by Bioz Stars, 2026-09
    99/100 stars
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